Hplc-ms Study of Phytoestrogens from Glycyrrhiza Glabra
نویسندگان
چکیده
In this paper we have analyzed the phytoestrogen content of Glycyrrhiza glabra roots harvested from four different locations in Syria. The presence of eight phytoestrogen compounds was simultaneously assessed through a LC-MS method, before and after hydrolysis. All tested extracts contain daidzein, daidzin, genistein, formononetin, ononin and coumestrol. Rezumat În această lucrare am analizat conţinutul de fitoestrogeni din rădăcinile de Glycyrrhiza glabra, recoltate din patru locaţii diferite din Siria. Prezenţa a opt compuşi fitoestrogenici a fost simultan evidenţiată printr-o metodă LC-MS, înainte şi după hidroliză. Toate extractele testate conţin: daidzeină, daidzină, genisteină, formononetină, ononină şi cumestrol.
منابع مشابه
Chemistry of phenolic compounds of licorice (Glycyrrhiza species) and their estrogenic and cytotoxic activities*
The genus Glycyrrhiza consists of about 30 species in which G. glabra, G. uralensis, G. inflata, G. aspera, G. korshinskyi, and G. eurycarpa are generally recognized as licorice because of their sweet taste. Except G. korshinskyi, we examined isoprenoid-substituted phenols of these licorices. Each plant could be characterized by some isoprenoid phenols. We also investigated the biological activ...
متن کاملDetermination of 18 ?-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC
A high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and UV detection at 230 nm. The...
متن کاملDetermination of 18 ?-Glycyrrhetinic Acid in Glycyrrhiza glabra L. Extract by HPLC
A high performance liquid chromatography method was studied for determination of 18 ?-glycyrrhetinic acid in Glycyrrhiza glabra L. (licorice) extract. The operating condition were C-18 reversed phase column (VP-ODS, (250×4.6 mm, 5 mm)) at room temperature, acetonitril/phosphoric acid (3/1) as mobile phase, at flow rate of 0.6ml/min (0-8min), 0.4ml/min (8-20min) and UV detection at 230 nm. The...
متن کاملField survey of Glycyrrhiza plants in Central Asia (1). Characterization of G. uralensis, G. glabra and the putative intermediate collected in Kazakhstan.
The characteristics of Glycyrrhiza plants from 12 collection sites in southeastern Kazakhstan were investigated. G. uralensis was observed at 9 of the sites from Almaty to Shu, and G. glabra was observed at 8 sites. At 4 sites near Shu, and 1 site near Almaty, G. glabra and G. uralensis grew together forming a mixed population, and intermediate-type plants between them were also observed at 3 s...
متن کاملDetermination of Glycyrrhetic Acid in Rat Plasma by HPLC After Oral Administration of Licorice Aqueous Extract
Licorice is obtained from Glycyrrhiza glabra (G. glabra) in Iran. Glycyrrhizin is the main constituent of G. glabra and has several pharmacological effects. It is hydrolyzed to glycyrrhetic acid (GA) in the intestine after oral administration. In this study, a novel HPLC method was used to determine the concentration of GA in rat plasma after oral administration ...
متن کامل